rospedРоссийский педиатрический журнал имени М.Я. СтуденикинаM.Ya. Studenikin Russian Pediatric JournalФГАУ «НМИЦ здоровья детей» Минздрава России10.46563/1560-9561-2023-26-2-89-94ssjkbvrosped-16Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL INVESTIGATIONSПолиморфизмы генов ренин-ангиотензиновой системы: значение в прогрессировании хронической болезни почек у детейPolymorphisms of genes of the renin-angiotensin system: significance in the progression of chronic kidney disease in childrenhttps://orcid.org/0000-0003-2864-6885СедашкинаОльга АлександровнаSedashkinaOlga A.

Канд. мед. наук, ассистент каф. факультетской педиатрии ФГБОУ ВО «Самарский государственный медицинский университет» Минздрава России

e-mail: sedashkina@inbox.ru

MD, PhD, Assistant of the Department of faculty pediatrics of the Samara State Medical University of the Ministry of Health of the Russian Federation, Samara, 443099, Russian Federation

e-mail: sedashkina@inbox.ru

sedashkina@inbox.ruhttps://orcid.org/0000-0002-3131-1368ПорецковаГалина ЮрьевнаPoretskovaGalina Yu.

Доктор мед. наук, доцент. зав. каф. факультетской педиатрии ФГБОУ ВО СГМУ Минздрава России

e-mail: g.yu.poreckova@samsmu.ru

gmakovetskaya@yandex.ruhttps://orcid.org/0000-0003-3934-8699МаковецкаяГалина АндреевнаMakovetskayaGalina A.

Доктор мед. наук, проф. каф. госпитальной педиатрии ФГБОУ ВО СГМУ Минздрава России

e-mail: gmakovetskaya@yandex.ru

g.yu.poreckova@samsmu.ruФГБОУ ВО «Самарский государственный медицинский университет» Минздрава РоссииSamara State Medical University of the Ministry of Health of the Russian Federation2023090820232328994Copyright © Седашкина О.А., Порецкова Г.Ю., Маковецкая Г.А., 20232023Седашкина О.А., Порецкова Г.Ю., Маковецкая Г.А.Sedashkina O.A., Poretskova G.Y., Makovetskaya G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rosped.ru/jour/article/view/16Введение

Введение. Комплексный анализ полиморфизмов генов ренин-ангиотензиновой системы (РАС) у детей с разными нозологическими формами нефропатий является необходимым этапом определения клинико-генетических особенностей формирования хронической болезни почек (ХБП).

Цель

Цель: установить особенности полиморфизмов генов АСЕ (D/I), AGT (Thr174Met), AGT (Met235Thr) и AGTR1 (A1166C) у детей с ХБП и определить их значение в прогрессировании заболевания.

Материалы и методы

Материалы и методы. В ретроспективное и проспективное когортное исследования включены 100 детей 1–17 лет с различными формами патологии почек, обследованные в течение 10 лет. Всем больным проведена идентификация однонуклеотидных полиморфизмов генов с помощью аллель-специфичной полимеразной цепной реакции амплификации с использованием тест-систем. Оценка клинико-параклинических маркеров прогрессирования ХБП проводилась 2 раза в год.

Результаты

Результаты. При патологии почек у детей выявлена тенденция к увеличению частоты встречаемости однонуклеотидных полиморфизмов генов, влияющих на активность РАС. У пациентов с ХБП 3–5 стадий, которые составили 35%, аллели D/D АСЕ сочетались с аллелями AGT Thr174Met (27,9 ± 6,83%) и Met235Thr (41,86 ± 7,5%), Thr235Thr (30,2 ± 7,0%) и AGTR1 A1166C (37,2 ± 7,32%) чаще, чем при лёгкой степени ХБП (0 и 7,5 ± 3,37; 5,2 ± 2,94 и 5,2 ± 2,94% соответственно; р ≤ 0,010).

Заключение

Заключение. Определение клинико-генетических особенностей ХБП необходимо для выделения групп риска и своевременного проведения превентивных мероприятий.

Участие авторов

Участие авторов:Седашкина О.А. — концепция и дизайн исследования; Седашкина О.А., Порецкова Г.Ю. — сбор и обработка материала, статистическая обработка материала, написание текста; Маковецкая Г.А. — редактирование. Все соавторы — утверждение окончательного варианта статьи, ответственность за целостность всех частей статьи.

Финансирование

Финансирование. Исследование не имело финансовой поддержки.

Конфликт интересов

Конфликт интересов. Авторы заявляют об отсутствии конфликта интересов. 

Поступила 16

Поступила 16.02.2023Принята к печати 21.03.2023Опубликована 28.04.2023

Introduction

Introduction. A comprehensive analysis of the polymorphisms of the genes of the renin-angiotensin system in children with different nosological forms of nephropathies is a necessary step in determining the clinical and genetic features of the formation of chronic kidney disease (CKD).

Aim

Aim: to establish the features of ACE (D/I), GT (Thr174Met), AGT (Met235Thr) and AGTR1 (A1166C) gene polymorphisms in CKD children and determine their significance in the progression of the disease.

Materials and methods

Materials and methods. A retrospective and prospective study included one hundered 1 to 17 years children with nephropathies, examined in the children’s nephrology department of the Samara Regional Hospital over 10 years. In children, the identification of single nucleotide polymorphisms of genes was carried out using an allele-specific polymerase chain amplification reaction using test systems. Evaluation of clinical and paraclinical markers of progression in CKD was carried out twice a year. The results of the study were evaluated with the calculation of the Student–Fisher criteria and correlation analysis.

Results

Results. in patients with kidney diseases, there was a trend towards an increase in the occurrence of single nucleotide polymorphisms of genes that affect the renin-angiotensin system (RAS). CKD patients at the stage 3–5 accounted for 35%. They had D/D ACE alleles combined with alleles AGT Thr174Met (27.9 ± 6.83%) and Met235Thr (41.86 ± 7.5%), Thr235Thr (30.2 ± 7.0%) and AGTR1 A1166C (37.2 ± 7.32%) more often than in milder CKD (0 and 7.5 ± 3.37%; 5.2 ± 2.94% and 5.2 ± 2.94%; respectively, p ≤ 0.010).

Соnclusion

Соnclusion. The study of clinical and genetic features of CKD is relevant for the purpose of timely implementation of preventive measures.

Contribution

Contribution: Sedashkina O.A. — concept and design of the study; Sedashkina O.A., Poretskova G.Yu. — collection and processing of material, statistical processing of material, writing the text; Makovetskaya G.A. — editing.All co-authors — approval of the desired version of the article, responsibility for the preservation of all parts of the article.

Acknowledgement

Acknowledgement. The study had no sponsorship.

Conflict of interest

Conflict of interest. The authors declare no conflict of interest.

Received

Received: February 16, 2023Accepted: March 21, 2023Published: April 28, 2023

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The authors declare that there are no conflicts of interest present.