rospedРоссийский педиатрический журнал имени М.Я. СтуденикинаM.Ya. Studenikin Russian Pediatric JournalФГАУ «НМИЦ здоровья детей» Минздрава России10.46563/1560-9561-2024-27-3-168-172wipoehrosped-706Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL INVESTIGATIONSИзменения экспрессии белка CDX2 и цитокератинов CK20, CK7 в слизистой оболочке желудка у школьников с Helicobacter pylori-ассоциированным гастритомChanges in the CDX2 protein and cytokeratins CK20, K7 expression in the gastric mucosa in schoolchildren with Helicobacter pylori-associated gastritishttps://orcid.org/0000-0002-1410-8747ВшивковВиталий АлексеевичVshivkovVitaliy A.

Канд. мед. наук, ст. науч. сотр. клинического отделения патологии пищеварительной системы у взрослых и детей «НИИ медицинских проблем Севера» — обособленное подразделение ФГБНУ «Федеральный исследовательский центр «Красноярский научный центр» СО РАН» e-mail: vitali1983@mail.ru

vitali1983@mail.ruhttps://orcid.org/0000-0003-3842-9147ПоливановаТамара ВладимировнаPolivanovaTamara V.

Доктор мед. наук, гл. науч. сотр., клиническое отделение патологии пищеварительной системы у взрослых и детей «НИИ медицинских проблем Севера — обособленное подразделение ФГБНУ «Федеральный исследовательский центр «Красноярский научный центр» СО РАН»

e-mail: tamara-polivanova@yandex.ru

MD, PhD, DSci., Chief scientist, Clinical division of pathology of digestive system in adults and children, Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences — Scientific Research Institute for Medical Problems of the North, Krasnoyarsk, 660022, Russian Federation

e-mail: tamara-polivanova@yandex.ru

tamara-polivanova@yandex.ruНаучно-исследовательский институт медицинских проблем Севера — обособленное подразделение ФГБНУ Федеральный исследовательский центр «Красноярский научный центр» Сибирского отделения Российской академии наукScientific Research Institute for Medical Problems of the North of Federal Research Centre «Krasnoyarsk Scientific Centre» Siberian Division of Russian Academy of Sciences202407082024273168172Copyright © Вшивков В.А., Поливанова Т.В., 20242024Вшивков В.А., Поливанова Т.В.Vshivkov V.A., Polivanova T.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rosped.ru/jour/article/view/706Введение

Введение. Влияние таких канцерогенных факторов, как бактериальная инвазия Helicobacter pylori, на экспрессию цитокератинов CK20, CK7 и распределение белка CDX2 в эпителии слизистой оболочки (СО) желудка и патофизиологическая значимость этих процессов изучены недостаточно.

Цель

Цель: определить изменения распределения белка CDX2 и экспрессии цитокератинов CK20, CK7 в СО желудка у школьников с H. pylori-ассоциированным гастритом.

Материалы и методы

Материалы и методы. Проведена эзофагогастродуоденоскопия с биопсией СО желудка у 89 детей в возрасте 7–17 лет с H. pylori-ассоциированным гастритом. Диагноз подтверждён морфологическим методом, определено наличие инфицирования H. pylori. Иммуногистохимически исследованы биоптаты для выявления белков CDX2, CK20, CK7 в эпителии СО желудка.

Результаты

Результаты. Выраженная экспрессия CK7 чаще выявлялась в СО тела желудка школьников 7–11 лет при H. pylori-ассоциированном гастрите по сравнению с обследованными без H. pylori (p = 0,003) и с инфицированными школьниками 12–17 лет (p = 0,017). Установлены различия экспрессии СК20 в СО антрального отдела желудка, которая у неинфицированных девочек была ниже, чем у девочек с бактериальной инвазией (p = 0,024). Также у девочек с H. pylori в СО тела желудка была выше экспрессия CK7 (p = 0,045). Отмечено усиление среди неинфицированных детей экспрессии CK20 в СО антрального отдела у мальчиков в сравнении с девочками, в большей степени это касалось старшей возрастной группы (p = 0,006). Усиление экспрессии CK7 у школьников младшей возрастной группы прежде всего касалось инфицированных H. pylori девочек в обоих отделах желудка. В СО тела желудка выявлен также самый высокий уровень CK7 при H. pylori-ассоциированном гастрите у девочек 7–11 лет (p = 0,004). Ассоциации белка CDX2 с инвазией H. pylori не установлено.

Заключение

Заключение. При H. pylori-ассоциированном гастрите у школьников в эпителии СО желудка установлена экспрессия CK20, CK7 и CDX2, её выраженность тесно связана с полом возрастом пациентов.

Участие авторов

Участие авторов:Вшивков В.А., Поливанова Т.В. — концепция и дизайн исследования, сбор и обработка материала, редактирование;Вшивков В.А. — статистическая обработка;Поливанова Т.В. — написание текста.Все соавторы — утверждение окончательного варианта статьи, ответственность за целостность всех частей статьи.

Финансирование

Финансирование. Исследование не имело финансовой поддержки.

Конфликт интересов

Конфликт интересов. Авторы заявляют об отсутствии конфликта интересов.

Поступила 06

Поступила 06.05.2024Принята к печати 11.06.2024Опубликована 12.07.2024

The effect of carcinogenic factors such as bacterial invasion of Helicobacter pylori on the expression of CK20, CK7 cytokeratins and the distribution of CDX2 protein in the epithelium of the gastric mucosa (CO) and the pathophysiological significance of these processes have not yet been sufficiently studied. Aim: to determine changes in the distribution of CDX2 protein and the expression of cytokeratins CK20, K7 in the gastric mucosa in schoolchildren with Helicobacter pylori-associated gastritis. To evaluate the association between the expression of CDX2 and cytokeratins CK20, CK7 in the epithelium of the gastric mucosa in schoolchildren with gastritis under conditions of bacterial invasion by H. pylori.

Materials and methods

Materials and methods. Esophagogastroduodenoscopy was performed with taking biopsies from the gastric mucosa in eighty nine 7–17 years children with gastroenterological complaints. The morphological method confirmed the diagnosis of gastritis and determined the presence of H. pylori infection. Biopsies were examined immunohistochemically to identify the proteins CDX2, CK20, CK7 in the gastric epithelium.

Results

Results. A more pronounced expression of CK7 was noted in the body of the stomach with H. pylori-associated gastritis in 7–11 year schoolchildren, in comparison with those examined without H. pylori (p = 0.003) and with infected 12–17 years schoolchildren (p = 0.017). There were were revealed differences in the expression of CK20 in the antrum, which were absent in uninfec­ted girls and were lower than in girls with bacterial invasion (p = 0.024). Also in girls with H. pylori and in the body of the stomach, CK7 expression was higher (p = 0.045). Among uninfected patients, there was an increase in CK20 expression in the antrum in boys compared to girls, and to greater extent, this concerned the older age group (p = 0.006). While the increased expression of CK7 in schoolchildren of the younger age group primarily affected girls infected with H. pylori in both parts of the stomach. In the body of the stomach, the highest level of CK7 was also observed in H. pylori-associated gastritis 7–11 years old girls (p = 0.004). The association of CDX2 with H. pylori invasion has not been established.

Conclusion

Conclusion. Thus, in schoolchildren with gastritis, there was detected an expression of CK20, CK7 and CDX2 in the epithelium of the gastric mucosa, with features of its severity and a close association with the gender and age characteristics of the child.

Contribution

Contribution:Vshivkov V.A., Polivanova T.V. — concept and design of the study, data collection and processing, editing the text;Vshivkov V.A. — statistical processing of the data;Polivanova T.V. — writing the text.All co-authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Acknowledgment

Acknowledgment. The study had no sponsorship.

Conflict of interest

Conflict of interest. The authors declare no conflict of interest.

Received

Received: May 06, 2024Accepted: June 11, 2024Published: July 12, 2024

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The authors declare that there are no conflicts of interest present.